Type: Package
Package: freqpcr
Title: Estimates Allele Frequency on qPCR DeltaDeltaCq from Bulk
        Samples
Version: 0.4.0
Authors@R: c(
    person(given="Masaaki", family="Sudo", email="masaaki@sudori.info", role=c("aut", "cre"), comment=c(ORCID="0000-0001-9834-9857"))
    )
Maintainer: Masaaki Sudo <masaaki@sudori.info>
Description: Interval estimation of the population allele frequency from qPCR analysis based on the restriction enzyme digestion (RED)-DeltaDeltaCq method (Osakabe et al. 2017, <doi:10.1016/j.pestbp.2017.04.003>), as well as general DeltaDeltaCq analysis. Compatible with the Cq measurement of DNA extracted from multiple individuals at once, so called "group-testing", this model assumes that the quantity of DNA extracted from an individual organism follows a gamma distribution. Therefore, the point estimate is robust regarding the uncertainty of the DNA yield.
URL: https://github.com/sudoms/freqpcr
Depends: R (>= 3.6), cubature
Imports: methods
License: GPL (>= 3)
Encoding: UTF-8
RoxygenNote: 7.1.1
Suggests: knitr, rmarkdown, remotes
VignetteBuilder: knitr
NeedsCompilation: no
Packaged: 2022-01-26 10:57:50 UTC; masaa
Author: Masaaki Sudo [aut, cre] (ORCID:
    <https://orcid.org/0000-0001-9834-9857>)
Repository: CRAN
Date/Publication: 2025-11-08 18:27:41 UTC
Built: R 4.6.0; ; 2026-04-25 19:04:10 UTC; unix
