R: Melt Curve Fitting and Melt Shift Analysis

# Melt Curve Fitting and Melt Shift Analysis

## Documentation for package ‘InflectSSP’ version 1.6

- DESCRIPTION file
- User guides, package vignettes and other documentation

## Help Pages

Correction | This function corrects the normalized abundance of each protein using a correction constant that is calculated in this function. The correction constant is determined using the difference between actual and predicted fit at the proteome level.
CurveFit1 | This function determines the 4 parameter or 3 parameter log fit for the proteome level curve.
CurveFit2 | This function determines the best curve fit for each protein using the data post correction and also determines the R squared for each curve fit
Import | This function imports data that will be analyzed in downstream functions.
InflectSSP | This function is the primary function that calls other functions in the program.
MeltCalc | This function determines melt shifts for all proteins that meet quality criteria and also determines the melt shift p-values
Normalize | This function normalizes the abundance values to that measured at the lowest temperature
Quantify | This function determines the median abundance value across the proteome for all experiments together
ReportDataMelts | This function generates results from the Inflect function after applying criteria input from the user
ReportSTRING | This function generates a STRING based network using the significant melt shifts from analysis