GC                      Calculate G and C content of nucleotide
                        sequences
Tm_GC                   Calculate the melting temperature using
                        empirical formulas based on GC content
Tm_NN                   Calculate melting temperature using nearest
                        neighbor thermodynamics
Tm_Wallace              Calculate the melting temperature using the
                        'Wallace rule'
c2s                     convert a vector of characters into a string
check_filter            Check and filter invalid base of nucleotide
                        sequences
chem_correction         Corrections of melting temperature with
                        chemical substances
complement              complement and reverse complement base of
                        nucleotide sequences
print.TmCalculator      Prints melting temperature from a
                        'TmCalculator' object
s2c                     convert a string into a vector of characters
salt_correction         Corrections of melting temperature with salt
                        ions
